|
Index:
Introduction
Stability Trials in Feeds(carried out at Rhodia
Food, Woodley, UK)
INTRODUCTION
A trial with the enzyme preparation ROVABIO™ EXCEL LC,
identified as endo-1,3(4)-ß-glucanase and endo-1,4-ß-xylanase
from Penicillium funiculosum has been done to check
the stability of these two enzymes at different temperatures.
The enzyme preparation ROVABIO™ EXCEL LC was maintained
at 3 different temperatures 10, 20 and 30°C (50, 68 and
86°F) from up to 26 weeks.
The results are summarized in the table below: (normalized
data)
| Active substance |
Batch n° |
Storage temperature
(°C), (°F)
|
Activity against elapsed
time (%)
(weeks)
|
|
0
|
4
|
8
|
12
|
26
|
|
Endo-1,3(4)-ß-glucanase
from
Penicillium funiculosum
|
WWE347 |
10, 50
20, 68
30, 86
|
100
100
100
|
100
98
93
|
106
100
97
|
105
100
99
|
99
99
93
|
|
Endo-1,4-ß-xylanase
from
Penicillium funiculosum
|
WWE347 |
10, 50
20, 68
30, 86
|
100
100
100
|
97
102
101
|
99
101
99
|
101
102
103
|
99
101
101
|
The endo-1,3(4)-ß-glucanase and endo-1,4-ß-xylanase
from Penicillium funiculosum with potassium sorbate
and sorbitol as stabilizer systems were stable at the 3 temperatures
over the period of 26 weeks.
For some application of spraying ROVABIO™ EXCEL LC onto
pellets, the product has to be diluted 1 in 5 with water.
We assayed the diluted ROVABIO™ EXCEL LC at 3 temperatures
10, 20, 30°C for up to 6 weeks.
The results are summarized in the table below: (normalized
data)
Batch n° /
dilution |
Storage temperature
(°C), (°F)
|
Activity against elapsed
time (%)
(weeks)
|
|
0
|
1
|
2
|
4
|
6
|
|
WWE347
1/5
|
10, 50
20, 68
30, 86
10, 50
20, 68
30, 86
|
100
100
100
100
100
100
|
98
95
90
99
95
96
|
80
83
84
94
98
97
|
89
90
87
100
102
98
|
93
92
88
102
103
104
|
There is no loss of endo-1,4-ß-xylanase activities
at the 3 temperatures; there has been a little decrease in
the endo-1,3(4)-ß-glucanase activities (10 % decrease).
It is concluded that the endo-1,3(4)-ß-glucanase and
endo-1,4-ß-xylanase activities from Penicillium funiculosum
are stable under normal conditions.
The stability data have been used to support the following
conditions of storage and shelf life:
ROVABIO EXCEL LC
| Storage |
For optimum stability, store product cool, between 2
and 10°C, and dry |
| Shelf life |
Product will maintain specified activity for 6 months
from date of analysis under optimum conditions. In addition,
it has been shown that product will maintain specified
activity for 6 months at < 30°C (86°F) from
the date of analysis. |
Enzyme Stability Trials in Feeds
(carried out at Rhodia Food, Woodley, UK)
Several different enzyme preparations were subjected to stability
trials in a formulated feed at different temperatures. The
enzyme preparations ROVABIO™ EXCEL LC and ROVABIO™
EXCEL AP are identified in this work as endo-1,3(4)-ß-glucanase
and endo-1,4-ß-xylanase from Penicillium funiculosum.
Products were maintained at 3 different temperatures: 20,
30 and 40°C (68, 86, 104°F) to enable the stability
to be measured over a period of 6 months. Ground pelleted
feed (obtained from Sun Valley Poultry) was used in this study.
| Active substance |
Batch n° |
Storage temperature
(°C), (°F)
|
Activity against elapsed
time (%)
(weeks)
|
|
0
|
4
|
9
|
13
|
26
|
|
Endo-1,3(4)-ß-glucanase
from
Penicillium funiculosum
|
WE043
WE313
MCBG/4 |
20, 68
30, 86
40, 104
20, 68
30, 86
40, 104
20, 68
30, 86
40, 104
|
100
100
100
100
100
100
100
100
100
|
107
111
117
99
95
98
119
90
97
|
98
96
92
84
80
114
120
104
78
|
113
112
109
94
78
45
97
98
88
|
96
100
82
95
94
59
100
94
56
|
|
Endo-1,4-ß-xylanase
from
Penicillium funiculosum
|
WWP095
WE313
WWP181 |
20, 68
30, 86
40, 104
20, 68
30, 86
40, 104
20, 68
30, 86
40, 104
|
100
100
100
100
100
100
100
100
100
|
96
108
99
63
84
140
75
138
98
|
119
113
15
71
93
117
65
161
87
|
119
114
115
86
101
85
106
135
162
|
88
107
96
99
96
101
103
91
89
|
There were some variations in the activities over a 6-month
period. This was associated mainly with the lack of complete
homogeneity in the enzyme feed preparation and variations
in the assays. The lack of complete homogeneity in the enzyme
feed preparations was a function of the method of preparation
of samples in the laboratory, and does not reflect the industrial
practice. The results show different enzyme active species
to be stable. The individual results are discussed below.
| Endo-1,3(4)-ß-glucanase from Penicillium
funiculosum |
The product is stable up to 40°C (104°F). There
was no significant loss of activity at 20, 30 and 40°C
(68, 86, 104°F)over 6 months. |
| Endo-1,4-ß-xylanase from Penicillium
funiculosum |
The product is stable up to 40°C (104°F). There
was no significant loss of activity at 20, 30 and 40°C
(68, 86, 104°F) over 6 months. |
|
